Neutrophil Cluster Quantification

Neutrophil Cluster Quantification

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Results on screen, not just in a file Quality checks on your data A workbook that explains itself How the calculation works

Results

Starting cluster size {{ results.summary.cluster_number }} • {{ results.elapsed_ms }} ms
Single-cell volume X
{{ results.summary.X | number:1 }}
± {{ results.summary.selection_sem | number:1 }}
The mean volume of the 20 reference measurements on the Selection sheet.
Cells in the field of view Y
{{ results.summary.Y | number:1 }}
± {{ results.summary.y_uncertainty | number:1 }}
The number of cells the field of view is estimated to contain.
Clusters found α
{{ results.summary.alpha | number:0 }}
 
How many retained objects were large enough to be assigned a cluster size of N or more.
Cells inside clusters β
{{ results.summary.beta | number:0 }}
{{ results.summary.clusterized_ratio_pct | number:1 }}% of the field
The assigned cluster sizes added together.
Clusters per 100 cells γ
{{ results.summary.gamma | number:2 }}
per 100 cells
The number of clusters as a percentage of the cells in the field.
Mean cluster size
{{ results.summary.mean_cluster_size | number:2 }}
largest {{ results.summary.max_cluster_size }}
Cells inside clusters divided by the number of clusters (β/α).

Worth knowing about this run

{{ check.title }} Warning For information
{{ check.message }}
{{ check.guidance }}

Where your objects went

Every row read from the Detailed sheet ends in exactly one of these four groups.

Group Rows Share What it means
{{ step.label }} {{ step.count | number:0 }} {{ step.pct | number:1 }}% {{ step.note }}
Rows read {{ results.accounting.rows_read | number:0 }} 100%

Object volumes, in multiples of one cell

Every retained object, sized against one cell. The dotted lines mark where the size assignment changes: objects between a pair of them are called that many cells.

{{ bar.title }}
Discarded as debris Retained, not clustered Counted as clusters

Objects sit on average {{ results.fit.offset_mean | number:3 }} of a cell away from a whole multiple, against {{ results.fit.baseline }} for volumes with no relationship to the single-cell volume at all. {{ results.fit.within_quarter_pct | number:0 }}% sit close to a whole number of cells. {{ results.histogram.overflow }} object(s) larger than the axis are not drawn.

Cluster size distribution

Cells per cluster Clusters Count Share Cells Cumulative
{{ row.size }} {{ row.clusters | number:0 }} {{ row.pct_clusters | number:1 }}% {{ row.cells | number:0 }} {{ row.cum_pct_clusters | number:1 }}%

If you had chosen a different starting cluster size

Starting size Clusters found (α) Cells inside clusters (β) Clusters per 100 cells (γ) Mean size
{{ row.n }} yours {{ row.alpha | number:0 }} {{ row.beta | number:0 }} {{ row.gamma | number:2 }} {{ row.mean_cluster_size | number:2 }}

The starting cluster size is a choice, not a measurement, and the figures above move with it. Quote the value you used wherever you report a result, and keep it the same across everything you compare.

The single-cell reference

Reference measurements {{ results.reference.selection_count | number:0 }} How many single-cell volumes the Selection sheet supplied. The method requires exactly 20.
Smallest reference volume {{ results.reference.selection_min | number:2 }} The smallest of the 20.
Median reference volume {{ results.reference.selection_median | number:2 }} The middle of the 20.
Largest reference volume {{ results.reference.selection_max | number:2 }} The largest of the 20.
Reference spread {{ results.reference.selection_std | number:2 }} The standard deviation of the 20 reference volumes.
Reference variability {{ results.reference.selection_cv_pct | number:2 }}% The spread of the reference volumes as a percentage of their mean.

The workbook holds the full results table plus the accounting, quality checks, excluded rows and a glossary. The link is single use and expires in {{ ui.downloadSecondsLeft }}s. The link has been used. Run the analysis again if you need another copy. Run the analysis again to get a fresh download link.